【摘要】 目的研究本所转入人端粒酶逆转录酶基因的人骨髓基质细胞(hMSC-TERT细胞)的生物学特性。方法复苏hMSC-TERT细胞第30代,扩增,流式细胞仪检测细胞表面抗原;进行体外脂肪细胞、成骨细胞定向诱导,并分别进行油红O染色、碱性磷酸酶染色及von Kossa染色。结果hMSC-TERT细胞表面抗原检测CD34、CD45、CD44、CD106、CD166均为阴性;脂肪细胞定向诱导后油红O染色见胞浆内有红色脂肪颗粒,成骨细胞定向诱导后见胞浆内有碱性磷酸酶染色阳性黑褐色颗粒,von Kossa染色见有钙结节形成。结论hMSC-TERT细胞依然保留骨髓基质干细胞的自我更新特性及多向分化潜能。更多还原

【Abstract】 Objective To investigate the biological characteristics of human bone marrow stroma cells immortalized by human telomerase reverses(hMSC-TERT).Methods The 30th passage hMSCs-TERT were amplified after recovery of cryopreservation.The surface antigenic features of the cells were analyzed by flow cytometry.Differentiation induction of adipose cells and osteoblasts was performed in vitro.The induced hMSCs-TERT were examined by Oil red-O staining,alkaline phosphates staining and von Kossa staining.Results The hMSC-TERT was negative for CD34,CD45,CD44,CD106 and CD166.After the induction,accumulated cytoplasmatic red lipid droplets were clearly visible in the adipose cells by Oil red O staining,and alkaline phosphates-positive pitchy grains formed in the intracytoplasm and calcium nodes were found by von Kossa staining in the osteoblasts.Conclusion The hMSC-TERT still retains the self-renewing characteristics of bone marrow stromal stem cells and pluripotent differentiation potential.更多还原