【摘要】 目的:建立RP-HPLC法测定保健食品中3种蒽醌衍生物含量。方法:以XTerraTMRP18(3.9 mm×150 mm,5μm)为色谱柱,流动相:甲醇+含0.5%冰醋酸水溶液(75+25),检测波长:270 nm,流速:1 ml/min,柱温:25℃,外标法定量。结果:同时测定3种蒽醌衍生物大黄酸、大黄酚、大黄素的含量,其浓度分别在5.4~54、7.5~75和8.8~88μg/ml范围内呈良好的线性关系。方法的平均回收率分别为:107.3%、99.7%、101.1%,RSD为4.2%、6.8%、7.3%。结论:该方法简便、快速,测定结果准确可靠,重现性好,可用于保健食品中蒽醌含量的测定。更多还原

【Abstract】 Objective:To establish a RP-HPLC method for simultaneous determination of three kinds of anthraquinone derivatives in Health foods.Methods:Anthraquinone derivatives were determined by HPLC using XTerraTM RP18(3.9 mm×150 mm,5 μm),0.5% acetic acid-methanol(25-75),detective wave 270 nm,flow rate 1 ml/min and column temperature 25℃.Results:The linearity of Rhein,Chrysophanol,Emodin were good in the range of 5.4～54、7.5～75 and 8.8～88 μg/ml.The average recovery of Rhein was 107.3%,Chrysophanol 99.7%,Emodin 101.1%.Relative standard deviations(RSD) of them were 4.2%,6.8%,7.3%.Conclusion:This method is simple,rapid,accurate and reproducible for detection of anthraquinone in Health foods.更多还原