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测试片法快速检验食品中金黄色葡萄球菌的研究

Study on rapid detection of Staphylococcus aureus with testing slip method in food

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【作者】 陈德云陈国雄巫结冰梁玉萍胡伟宁张慧红

【Author】 Chen De-yun,Chen Guo-xiong,Wu Jie-bing,Liang Yu-ping,Hu Wei-ning,Zhang Hui-hong(Shunde Center dor Disease Control and Prevention,Foshan 528300,China)

【机构】 广东省佛山市顺德区疾病预防控制中心广东省佛山市顺德区疾病预防控制中心 广东佛山528300广东佛山528300

【摘要】 目的:快速检测食品中的金黄色葡萄球菌。方法:采用金黄色葡萄球菌测试片检验食品中的金黄色葡萄球菌,并用国标法进行对比。结果:测试片法检验金黄色葡萄球菌特异高,最小检出量0.11 cfu/g(m l),敏感度为1.2×10-8,比国标法可提高3个指数的最小检出量。阳性检出率可提高20.0%~25.5%。结论:测试片法操作简单,方便快捷,结果易观察。方法准确可靠,抑制杂菌能力强,工作周期短,可减少1/2的工作量。

【Abstract】 Objective:To detect Staphylococcus aureus in food rapidly.Methods:The detection result of Staphylococcus aureus in food by testing slip method was studied and compared with Chinese national standard method(GB).Results:Testing slip method was a high-specificity detection method for Staphylococcus aureus with the sensitivity of 1.2×10-8,and its minimum detectable quantity was 0.11 cfu/g(ml),which increased 3-log10 compared with Chinese national standard method(GB).Increase of positive detecting rate in this method was in the range of 20.0%~25.5%.Conclusion:The method is simple,convenient,accurate and stable.Moreover,the method with the short detecting period can reduce one half of the workload.

【关键词】 金黄色葡萄球菌测试片
【Key words】 Staphylococcus aureusTesting slip
  • 【文献出处】 中国卫生检验杂志 ,Chinese Journal of Health Laboratory Technology , 编辑部邮箱 ,2007年08期
  • 【分类号】R155.3
  • 【被引频次】12
  • 【下载频次】232
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