【摘要】 目的:了解本地区自然水体中霍乱弧菌(VC)的分布,对收集的O139群VC进行选择性抗生素耐药性标记的流行病学分析。方法:2000~2004年霍乱流行季节,使用改良免疫荧光菌球法检测黄浦江原水;使用改良K-B法检测收集到的O139群VC两类菌的耐药性。结果:5年间从1963件水样中共检出57株VC,总阳性率2.90%,除1株稻叶1d外其余皆为O1或O139群VC的非流行株;耐药谱显示2000年和2001年的O139病人株对氨基糖甙类、四环素类和呋喃唑酮存在显著性差异(P<0.05),而部分O139甲鱼株的耐药谱与病人的相近,提示可能为相同来源。结论:改良免疫荧光菌球法可以同时检测水样中的O1和O139群VC,具有可推广性;2000年和2001年O139病人株可能属于不同的克隆群,甲鱼是潜在的传染源。更多还原

【Abstract】 Objective:To realize the distribution of Vibrio cholerae(VC) in natural water of local area and collection of strains of O139 selectivity to carry out epidemiological analysis with antibiotic resistance mark.Methods:From 2000 to 2004,we detected raw water from Huangpu river with modified immunofluorescence colonies method and used modified K-B method to detect antibiotic resistance of characterization differences of O139 strains stock.Results:The 57 strains of VC was detected from 1963 river samples for 5 years,total positive rate was 2.90%,that belonged to O1 Eltor and O139 serogroups non-epidemic strains except 1 Eltor inaba 1d.Antibiotic resistance spectrum showed O139 patient-isolated strains from 2000 to 2001 had significant difference between aminoglycosides,tetracyclines and furazolidone(P<0.05).The antibiotic resistant rates of some O139 turtle-isolated strains was similar to the patient-isolated′s,which showed it had the same source.Conclusion:Modified immunofluorescence colonies method could be used for detection of O1 and/or O139 VC from row water samples together and of great worth.O139 patient-isolated strains from 2000 to 2001 may belong to different clones and turtle is potential contaminant.更多还原