【摘要】 目的:在64只大鼠上,观察了侧脑室(icv)注射γ-氨基丁酸(GABA)后对束旁核(PF)痛兴奋神经元(PEN)电活动的影响。方法:采用电生理学方法,以电脉冲刺激右侧坐骨神经作为伤害性刺激,用玻璃微电极细胞外记录PF内PEN放电。结果:(1)伤害性刺激可使PF内PEN痛诱发放电频率增加;(2)icv注射GABA(500μg/10μl)抑制PEN的电活动,使PEN诱发放电频率减少,潜伏期延长;(3)这种作用可被icv注射GABAA受体拮抗剂荷包牡丹碱(B ic,10μg/10μl)所阻断。结论:icv注射GABA可使大鼠PF内PEN对伤害性刺激的反应减弱,GABAA受体参与介导这种镇痛作用。更多还原

【Abstract】 Objective:To observe the effects of γ-aminobutyric acid(GABA)on the electric activities of pain-excitation neurons(PEN)in nucleus parafascicularis(PF) in 64 rats.Methods: The trains of electric impulses applied to the right sciatic nerve were used as noxious stimulation.The discharges of PEN in PF were recorded extracellularly with glass microelectrodes.Results:(1)The noxious stimulation increased the frequency of evoked discharges of PEN.(2)The intracerebroventricular(icv)injection of GABA(500 μg/10μl)inhibited the electric activities of PEN,decreased the frequency of evoked discharges and prolongated the latency of PEN.(3)The effects could be blocked by the icv injection of bicuculline(Bic,10 μg/10μl),GABAA receptor antagonist.Conclusion:The response of PEN in PF of rats to the noxious stimulation was weakened by the icv injection of GABA.The GABAA receptor participated in mediating the analgesic effects.更多还原