【摘要】 本研究应用重组抗原,成功建立了检测猪伪狂犬病病毒血清抗体的间接ELISA诊断方法。通过方阵滴定确定了抗原最适包被量为1.45μg/孔,血清最适稀释度为1∶80,其作用时间为60 min,酶标抗体最适作用时间为60 min。其阳性临界值为OD≥0.123。此外,该抗原不与猪其他疾病的阳性血清反应,具有良好的特异性;批内重复试验,变异系数小于10%。该方法与进口诊断试剂盒的符合率为96.3%。本研究为现地免疫猪群抗体检测和进行PRV流行病学调查提供了一种简便的血清学诊断方法。更多还原

【Abstract】 We utilized recombinant antigen and developed an indirect ELISA assay for detecting PRV antibodies.The optimal coating concentration of antigen was 1.45 μg of PRV protein perwell.The dilution of sera was 1∶80.The reaction time of sera was 60 min.The reaction time of conjugate was 60 min.The standard of judgment was that OD≥0.123 was positive.Furthermore,the recombinant antigen had no crossreaction with other sera of swine diseases,and it showed this assay was highly specific.In the intro-batch duplicativity test,the variation coefficient is less than 10%.The result showed the assay had good replicativity.When compared with the commercial Kit,96.3% agreement was obtained.The application of indirect ELISA assay would provide a simple and rapid means of detecting anti-PRV in monitoring PRV infection or assessing vaccination in the field.更多还原