【摘要】 根据胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)各血清型之间外毒素apxⅠ、apxⅡ、apxⅢ、apxⅣ A基因差异,设计6对引物,对16株标准菌株进行1次5对引物的多重PCR和1次根据apxⅣ A基因设计1对引物PCR的扩增,得到各血清型特异性片段,并将片段特征相同的血清型归为同一组。通过这2步PCR分组情况的不同能将16株标准菌株中的大多数血清型区别开,但是仍然不能将血清2型和8型、血清9型和11型、血清5型中的2个亚型以及血清12型和13型区分开。又根据血清2型荚膜多糖(CP)基因差异设计1对引物将血清2型和8型区分开。将此分型系统应用于23株未知病原菌检测,检测到胸膜肺炎放线杆菌9株,并全部能够将其分型。本试验PCR分型体系可以作为胸膜肺炎放线杆菌血清型分型的一种快速而有效的鉴定方法。更多还原

【Abstract】 Six pairs of primers were designed on the basis of apxⅠ、apxⅡ、apxⅢ、apxⅣA exotoxin genes for serotyping Actinobacillus plueropneumoniae, A multiplex PCR using the five pairs of primers and a single PCR based on the apxⅣA gene were performed. The results reveled that most of serotype of the reference strains of A.plueropneumoniae could be successfully differentiated. However, it was not possible to distinguish serotype 9 from 11, serotype 12 from 13 and serotype 2 from 8, then a PCR was developed to identify serotype 2 and 8 on the basis of conserved capsular polysaccharide of serotype 2. In order to evaluate the PCR typing system′s veracity, then the PCR typing system was further applied for detecting 23 known pathogens. 9 out of 23 know strains were identified and serotyped. The PCR results fully consist with the records. Thus, the PCR system may provide a rapid and useful tool for typing the serotypes of A. plueropneumoniae.更多还原