【摘要】 【目的】基因芯片技术建立颅内动脉瘤患者血液单核细胞基因表达谱,筛选与信号传导相关的基因表达情况。【方法】50例颅内动脉瘤患者外周血,10例正常成人作为对照组,分离血液单核细胞,提取总RNA,与含22215个人类基因的Affymetrix寡核苷酸基因芯片杂交、洗脱、染色和扫描,分析检测的数据。荧光定量RT-PCR验证芯片结果。【结果】检测的22215个基因中,颅内动脉瘤共差异表达的基因有325个,差异水平均达2倍以上的基因有35个,生物信息学分析发现信号传导相关基因有7个,其中上调基因5个,下调基因2个。【结论】血液单核细胞基因表达谱是研究颅内动脉瘤发病机制的新策略,多个信号传导相关基因参与颅内动脉瘤的形成和发展。更多还原

【Abstract】 [Objective] To identify signal transduction-related genes in peripheral blood mononuclear cells from intracranial aneurysm patients using oligo-microarrays.[Methods] Blood samples from 50 intracranial aneurysms and 10 healthy human donors were obtained after informed consent. Peripheral blood mononuclear cells (PBMC) were isolated from blood using Ficoll method. The cDNA were retrotranscribed from the extracted RNA and the biotin-labeled cRNA derived from the transcription of cDNA were fragmented as probes. Then, the probes were hybridized with Affymetrix Human Genome U133A Array. Gene Array Scanner was used to screen the signals of hybridization and Microarray Suite software 5.0 was applied to analyze the expression of genes. [Results] There were 325 genes differently expressed in PBMCs from intracranial aneurysm patients compared to healthy human. Function analysis were confirmed from 21 upregulated genes and 14 downregulated genes above 2-fold level. Among these genes, there are 7 genes invovled in signal transduction. [Conclusions] The methodologies of gene chip of PBMCs will provide a new powerful approach to identify the molecular mechanisms of intracranial aneurysm. Signal transduction may be the possible mechanism for the pathogenesis of intracranial aneurysms in Chinese.更多还原