【摘要】 采用反相高效液相色谱层析法(RP-HPLC)分离中华绒螯蟹(Eriocheir sinensis)眼柄视上神经节中的性腺抑制激素,收集在高血糖激素家族区域内出峰的洗脱液组分,得到7组单一的样品组分。通过活体注射的方法,观察和统计每一组分对去眼柄中华绒螯蟹卵母细胞直径大小的影响,以检验每个分离组分的性腺抑制活性。结果发现,其中的第7组分对中华绒螯蟹的卵巢发育具有最强的抑制作用,推测这一组分为中华绒螯蟹的性腺抑制激素。更多还原

【Abstract】 The gonad inhibiting hormone(GIH) is a member of Crustacean hyperglycemic hormone(CHH) peptide family.It plays a significant role in inhibiting development of crab’s gonad.In this research,the crude hormone extract of crab’s optic ganglia was further isolated by reverse-phase high-performance liquid chromatography(RP-HPLC).Seven single fractions were obtained.The biological activity of each fraction was assayed through injecting in vivo to the crabs whose optic ganglia were removed.After 6 days of injection the diameter of crab’s oocytes was recorded and statistically analyzed.The results showed that among the 7 isolated fractions,fraction 7 had the greatest biological activity to suppress the development of crab oocytes,which indicated that this fraction was the GIH of Eriooheir sinensis.更多还原