【摘要】 目的探讨MLVA技术在福建省结核分枝杆菌基因分型中的应用。方法选取12个分型效果较好的VNTR基因位点。应用聚合酶链反应(PCR)和琼脂糖凝胶电泳,建立检测结核分枝杆菌DNA指纹多态性的方法,分析结核分枝杆菌DNA多态性。结果共对105株结核分枝杆菌的12个VNTR位点进行了检测,根据这些菌株的指纹多态性特征,共分为4个基因型(I型I、I型I、II型I、V型),其中Ⅰ型所占比例最大,为66.7%(70/105),Ⅱ型占20%(21/105),Ⅲ型占9.5%(10/105),Ⅳ型占3.8%(4/105)。结论结果提示福建省结核分枝杆菌的传播是以Ⅰ型菌株为主,应加强此型菌株流行的监控。更多还原

【Abstract】 A new method based on the variable number tandem repeat(VNTR) analysis was applied for the genotyping of Mycobacterium tuberculosis,in which NNTRs from 12 loci with good genotyping efficiency were selected,and a rapid finger-printing method was established by PCR and agarose gel electrophoresis used to analyze the DNA polymorphism of drug-resistant M.tuberculosis.The polymorphism of the DNA finger-printing of M.tuberculosis was analyzed with BioNumerics software(Version 4.5).Based on the characteristics of the PCR-amplified finger-prints obtained,the 105 strains of M.tuberculosis isolated from Fujian province which were detected in 12 VNTR loci,could be classified into 4 genetic types,in which type 1 was the predominant genotype.The percentages of type 1,II,III and IV were 66.7%(70/105),20.0%(21/105), 9.5%(10/105) and 3.8%(4/105) respectively.These results indicate that the transmission of tuberculosis in Fujian province is mainly due to M.tuberculosis type I and the surveillance on this type of microorganism needs to be strengthened.更多还原