【摘要】 目的:探索¹³¹I标记表皮生长因子（EGF）对胶质瘤显像的可行性。方法:用神经胶质瘤C6细胞进行放射性摄取和滞留的体外实验;荷胶质瘤裸鼠尾静脉注射¹³¹I-EGF,于注射后30min、2h、6h、12h进行¹³¹I-EGF在鼠体内的放射性分布研究;在荷瘤鼠与正常对照鼠尾静脉注射¹³¹I-EGF后12h进行SPECT显像研究。结果:体外培养C6细胞对¹³¹I-EGF有较好的放射性摄取和滞留;在各个时间点,¹³¹I-EGF主要分布在荷瘤鼠肝、脾、肾等组织,瘤区放射性分布较高,脑组织最低,随时间延长肿瘤组织放射性分布逐渐增加,在12h时达最高;12h时SPECT显像图像清晰,瘤组织放射性分布明显,肝脾组织放射性分布较高,轮廓完整。结论:¹³¹I标记EGF有望为临床神经胶质瘤提供一种新的核医学诊断方法。更多还原

【Abstract】 Objective:To explore the feasibility of diagnosing glioma which expressing high level EGFR by 131I-EGF.Methods: In vitro cellular uptake and cellular retention experiment of ¹³¹I-EGF was carried out with C6 cell.Radiodistribution experiment was carried out after injection of ¹³¹I-EGF into 2-week-old tumor bearing Wistar rats,at 30min,2 hour,6 hour and 12 hour. SPECT images were carried out after 12 hours of intravenous injection of ¹³¹I-EGF between tumor bearing rats and normal rats. Results:The rate of C6 cellular uptake and retention in vitro was high.At all selected time,¹³¹I-EGF was mainly distributed in liver,spleen and kidney,partially distributed in tumor,but the radioactivity was stronger and stronger with the time passed,until reached the highest strong at 12h.Tumor tissues of tumor bearing rats were seen clearly by SPECT imaging after 12 hours of intravenous injection of ¹³¹I-EGF,the liver and spleen were the major normal organs visualized on the images,and the other tis- sues didn’t be seen obviously.Conclusions:¹³¹I-EGF could offer a new and specific approach to diagnose tumor such as glioma and other tumors expressing high level EGFR from molecular level.更多还原