【摘要】 目的:探讨低氧诱导因子1α(HIF-1α)对小鼠宫颈癌皮下移植瘤放射敏感性的影响,探讨其可能的机制。方法:建立宫颈癌皮下移植瘤模型,随机分为对照组、单次γ射线大剂量照射组、小剂量分次照射组、大剂量照射联合高压氧组、小剂量分次照射联合高压氧组,采用不同的方案进行体外放射治疗。免疫组化法测定放射治疗后移植瘤内的HIF-1α、bcl-2的表达水平,观察各γ射线处理组照射后移植瘤的抑瘤率,并对HIF-1α与bcl-2的表达及抑瘤率进行相关性分析。结果:HIF-1α的表达与移植瘤抑瘤率呈负相关(r=-0.514,P<0.05);与Bcl-2表达无明显相关关系。结论:抑制HIF-1α的表达可提高肿瘤对放射治疗的敏感性,该效应可能与Bcl-2表达无关。更多还原

【Abstract】 Objective:To study the effect of Hypoxia-inducible factor-1 alpha(HIF-1α)on the radiosensitivity of rat cervical carcinoma and discuss the possible mechanism.Methods:The transplantation tumor model of cervical carcinoma was established. 77 rats were randomly divided into five groups:control group,the large fraction irradiation group,the small fraction irradiation group,the large fraction irradiation combining HBO group and the low dose irradiation combining HBO group.Expressions of HIF-1α and bcl-2 in transplantation tumor after treatment were detected by immunohistochemical staining,and the tumor inhibitory rate by the change of tumor volume.The correlation between expressions of HIF-1 α and bcl-2(and tumor inhibitory rate)was also observed.Results:Expression of HIF-1 α and carcinoma growth inhibitory rate showed a reverse correlation(r=-5.147,P<0.05).No correlation was found between the expression of HIF-1 α and bcl-2.Conclusions:Inhibiting the HIF-1α expression could effectively promote the radiosensitivity of rat cervical carcinoma without impacting the expression of Bcl-2.更多还原