【摘要】 对近几年报道的磺化修饰策略及其应用进行了评述。通过磺化修饰在肽段上引入带负电荷的磺酸基,不仅可以诱导肽段离子的质谱裂解,得到以y系列离子为主的序列信息丰富的串联质谱图,有利于肽段从头测序,还可以改变被修饰肽段的电荷分布,通过离子交换色谱法分离富集磺化修饰的肽段,有助于蛋白质鉴定。更多还原

【Abstract】 Negatively-charged sulfonic group introduced by sulfonation derivatization not only induced the MS/MS fragmentation of peptides and produced an exclusive series of y-type ion spectrum,but also changed the charge distribution of sulfonated peptides and made the enrichment of target peptides by ion-exchange chromatography possible.This technique enormously facilitates de novo peptide sequencing and protein identification by mass spectrometry.The procedure of sulfonation derivatization and its application in the study of proteomics are reviewed.更多还原