【摘要】 目的建立C57BL/6J小鼠肝癌动物模型,为深入开展肝细胞癌的实验研究打下基础。方法取90只C57BL/6J小鼠,联合采用二甲基亚硝胺(DEN)/四氯化碳(CCl4)/乙醇诱导20周,观察其肝癌的发生情况。另取10只同种小鼠作为正常对照组。RT-PCR法检测病变肝组织中AFP基因表达。结果实验组90只小鼠共死亡8只;病理学检查发现,存活的82只小鼠中有71只发生肝癌,诱癌成功率为78.9%(71/90),肝癌小鼠的肝癌组织学类型以中、高分化为主;另11只有不同程度的药物中毒性肝炎或肝硬化(12.2%);对照组小鼠肝脏均未发现明显异常。成癌组中的肝癌组织RT-PCR检测可见AFP基因表达,非肝癌组织及对照组于组织中无AFP表达。结论采用DEN/CCl4/乙醇联合能诱导出C57BL/6J甲胎蛋白分泌型小鼠肝癌,诱导时间相对较短,癌变率高,部分病变肝组织中伴有肝炎、肝硬化病理学改变,是较理想的研究肝癌的实验动物模型。更多还原

【Abstract】 Objective To establish a stable primary hepatocellular carcinoma(HCC) model of C57BL/6J mice,and establish a basis for in-depth laboratory research in hepatocellular cancer.Methods Ninety C57BL/6J mice were induced to establish HCC models by combination of diethylnitrosamines(DEN),carbon tetrachloride(CCl4) and ethanol for twenty weeks,then the occurrence and development of HCC were observed,and other 10 C57BL/6J mice were used as normal control(no drug was given).RT-PCR method was used to observe AFP mRNA expression in liver tissues and tumor tissues.Results seventy-one of 90 mice developed HCC(78.9 %),cirrhosis was observed in 11 of 90 mice(12.2 %),and 8 of them died of toxic hepatitis and acute hepatocellular necrosis.AFP mRNA was expressed only in liver cancer tissues.No obvious liver pathologic changes were observed in the control group.The histo-pathologic changes of HCC were high or middle differentiation.Conclusions An AFP-secreting C57BL/6J mouse liver cancer model was established by induction with combination of DEN,CC14 and ethanol in a relatively short time.The rate of cancerous change was high,and some of the hepatic changes included hepatitis and cirrhosis.This is a rather ideal animal model for the study of liver cancer.更多还原