【摘要】 目的探讨乳腺癌转移抑制基因BRMS1 mRNA在乳腺癌组织中的表达及其临床意义。方法用逆转录聚合酶链反应(RT-PCR)技术检测71例乳腺癌、71例癌旁组织及12例乳腺良性肿瘤、12例正常乳腺组织中BRMS1 mRNA的表达,半定量分析RT-PCR产物电泳条带密度。结果在71例乳腺癌及其相应癌旁组织中BRMS1 mRNA表达水平分别为0.378±0.046和0.918±0.044;12例乳腺良性肿瘤及正常乳腺组织表达水平分别为0.908±0.047和0.934±0.028;乳腺癌组织BRMS1 mRNA的表达水平明显低于癌旁组织、乳腺良性肿瘤和正常乳腺组织(P<0.01)。BRMS1 mRNA的表达水平与患者年龄、肿瘤大小、ER和PR无关(P>0.05),而腋淋巴结有转移及临床分期III,IV期者其表达水平低于无淋巴结转移和临床分期I,II期者(P<0.05)。结论BRMS1 mRNA在乳腺癌组织中表达水平下降;其表达与乳腺癌腋淋巴结转移、临床分期有关,可能成为乳腺癌转移和预后的指标之一。更多还原

【Abstract】 Objective To study the expression of BRMS1mRNA in human breast cancer tissues and their significance.Methods The expression of BRMS1mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR) in 71 breast cancer tissues and adjacent breast tissues,12 patients with benign breast tumors and 12 patients with normal breast tissue,and semi-quantitative analysis of band densities was also performed.Results The expression of BRMS1mRNA in 71 patients with breast cancer and adjacent breast tissue was 0.378±0.046 and 0.918±0.044,respectively;the expression of BRMS1mRNA in 12 patients with benign breast tumors and 12 patients with normal breast tissue was 0.908±0.047 and 0.934±0.028 respectively.BRMS1mRNA expression was significantly lower in breast cancer tissue compared to adjacent breast tissue,benign breast tumors and normal breast tissue(P<0.01).The expression of BRMS1mRNA in breast cancer was reduced and had no relation with patient age,tumor size,and ER,PR(P>0.05),but was related to axillary lymph node metastasis and clinical stage(P<0.05).Conclusions The expression of BRMS1mRNA in patients with breast cancer was correlated with axillary lymph node metastasis and clinical stage,and the reduction of BRMS1mRNA expression could be an index for metastasis and prognosis of patients with breast cancer.更多还原