【摘要】 目的观察N-乙酰半胱氨酸(NAC)灌肠对小鼠急性溃疡性结肠炎的作用。方法5%葡聚糖硫酸钠(DSS)自由饮用7 d诱导小鼠急性溃疡性结肠炎,同时予以生理盐水(NS)、5-氨基水杨酸(5-ASA)、NAC保留灌肠,观察小鼠体重、粪便性状、隐血便血,计算疾病活动度(DAI)积分,检测结肠长度、结肠过氧化物酶(MPO)活性、血清过氧化物歧化酶(SOD)活力和丙二醛(MDA)含量及肠黏膜病理改变。结果NAC组小鼠隐血、便血、体重下降、DAI积分、病理改变等均好于模型组、NS组(P<0.05),与5-ASA组疗效相似。NAC组SOD活力高于模型组,MPO活性、MDA含量则低于模型组(P<0.05)。结论NAC对DSS诱导的小鼠急性溃疡性结肠炎黏膜损伤有保护作用,其机制可能与抗氧化应激有关。更多还原

【Abstract】 Objective To test the effect of N-acetylcysteine on the murine model of ulcerative colitis(UC) induced by dextran sodium sulphate.Methods UC was induced by adding 5% dextran sodium sulphate into the drinking water for 7 days.The mice of different groups were injiciatur enema with normal sodium,5-ASA,N-acetylcysteine respectively.We observed the body weight,feces description,hemafecia,colon length,disease activity scoring,myeloperoxidase activity,activities of superoxide dismupase,and content of malondialdehyde in the serum,and histological scoring.Results The body weight decreased.The colon length,disease activity scoring,myeloperoxidase activity,superoxide dismutase,malondialdehyde, and histological scoring of N-acetylcysteine group were better than those of DSS group and normal sodium group while similar to those of 5-ASA group.Conclusion N-acetylcysteine can protect the damage in dextran sodium sulphate-induced colitis in mice.One of the mechanisms may be anti-oxidative stress.更多还原