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生防菌BH-2发酵液中活性代谢产物的抑菌作用及理化性质
Study on Antifungal Action and Physicochemical Characteristics of Bioactive Metabolite Produced by Biocontrol Bacterial Strain BH-2 Fermentation
【摘要】 为了明确生防菌BH-2的抑菌作用和抑菌因子的相关性质,采用管碟法,对其发酵液中活性代谢产物的抑菌作用及理化性质进行了研究。结果表明,发酵液中活性代谢产物具有较广的抗菌谱,对多种植物病原菌均表现较好的抑制作用。此外,活性代谢产物耐121℃高温,在偏酸性或中性条件下稳定,有较强的抗紫外线照射能力,对蛋白酶K和胰蛋白酶稳定性较好,54℃下15d的稳定性较好。这为该菌株的进一步研究和产品开发奠定了坚实的基础。
【Abstract】 A study on antifungal action and physicochemical characteristics of bioactive metabolite produced by biocontrol bacterial strain BH-2 fermentation was launched by means of cylinder plate method for the sake of evaluating the antifungal action and relative properties of inhibitive factors of strain BH-2.The results showed that the bioactive metabolite had a broad antifungal spectrum and exhibited antagonistic abilities to several plant pathogens.In addition,it could endure the treatment of a high temperature of 121℃,was stable at acidic,neutral,54℃ for 15d respectively,maintained the antifungal activity after treatment of proteinase K,trypsin and ultraviolet radiation.These provided substantial foundation for a further research and production development of strain BH-2.
【Key words】 Biocontrol bacterium BH-2; Bioactive metabolite; Antifungal activity; Physicochemical characteristics;
- 【文献出处】 中国农学通报 ,Chinese Agricultural Science Bulletin , 编辑部邮箱 ,2007年09期
- 【分类号】S476.1
- 【被引频次】17
- 【下载频次】264