【摘要】 稻瘟病菌SSR检测是分子标记辅助育种的一项重要技术。为优化SSR反应体系,以稻瘟病菌菌株504为供试材料,采用单因素筛选法及L9（34）正交试验设计,研究了稻瘟病菌SSR分析中PCR反应体系的主要成分对扩增结果的影响。结果表明:在总体积为20μl的PCR反应中,TaqDNA聚合酶的最适用量为1.0U;Mg²⁺、dNTP和引物的最适终浓度分别为1.0mmol/L、100μmol/L和0.4μmol/L。利用该体系进行扩增,所得谱带清晰、稳定、非特异性带少。更多还原

【Abstract】 SSR detection is an important technique of molecular marker for assisted breeding in Pyricularia grisea. In order to optimize SSR-PCR reaction system, the main factors affecting amplication results were studied with single factor selection and L9(34) orthogonal design. The results showed that the optimal reaction system was as follows: the 20μl reaction system contained TaqDNA polymerase 1.0U, Mg2+ 1.0 mmol/L, dNTP 100 μmol/L and Primer 0.4μmol/L. Using this system, amplication bands were clear, standard and non-especial bands were shorter.更多还原