【摘要】 采用酸化和加热相结合的方法,研究了从黑麦草中提取叶蛋白的最佳工艺条件,并分析了黑麦草叶蛋白中的氨基酸组成。结果表明黑麦草叶蛋白提取的最佳工艺条件为pH4.0,料水比1:2,凝聚温度为70℃,加热时间5min。该条件下提取的叶蛋白含量可高达70.48%。氨基酸分析显示黑麦草叶蛋白中人体必需氨基酸、鲜味氨基酸和药效氨基酸分别占总氨基酸的40.3247%、22.2439%和77.5609%。黑麦草叶蛋白具有很好的营养、保健和药用价值。更多还原

【Abstract】 The optimum conditions of extracting LPC from the fresh leaves of Ryegrass were studied by using acid-heat methods. And the amino acid contents of LPC were determined. The results show that the optimum extraction technique of LPC was as pH 4.0, the ratio 1:2 for fresh leaf to water and 70℃ with 5min. In such condition the protein concentration of LPC can reach 70.48%. The proportion of essential amino acid, sapor amino acid and drug-effective amino acid in total amino acid are 40.3247%, 22.2439% and 77.5609% separately. The LPC from ryegrass have nutritional, healthy and drug-effective value.更多还原