【摘要】 【目的】研究N,O-羧甲基壳聚糖对鸡的急性毒性,评价该药的安全性;【方法】以最大浓度的N,O-羧甲基壳聚糖(10.5%),按20ml/(kg·bw)(2.1g/(kg·bw)/次)剂量24h内给鸡灌服;【结果】给药后连续观察7天,各组试验鸡全部存活,临床、剖检均未见到异常变化,测不出LD50,根据新药审批办法中关于急性毒性的要求,进行最大耐受量的测定。鸡灌服给予N,O-羧甲基壳聚糖的最大耐受量为10.5g/(kg·bw),相当于临床拟用量的3500倍;【结论】N,O-羧甲基壳聚糖毒副作用很小,临床可以安全应用。更多还原

【Abstract】 [Objective]To investigate the acute oral toxicity of N,O-carboxymethyl chitosan in chicken and evaluate the security of the drug;[Method]chickens were given 10.5% N,O-carboxymethyl chitosan 20ml/(kg·bw) (2.1g/(kg·bw) per time) orally within 24 hours, which was the maximum concentration and volume that could be allowed;[Results]Following exposure, animals were observed for a further 7 days, all the animals survived and no abnormal clinical signs were observed. At the end of the treatment, all surviving animals were necropsied and also no macroscopic effects on organs or tissues were observed. LD50 couldn’t be determined. Therefore, according to the requirements of the new drug method of examination and approval, MTD was determined. The maximal tolerance dose of N,O-carboxymethyl chitosan given orally was 10.5g/(kg·bw), which was 3500 times higher than its clinical recommended dose;[Conclusion]In broiler chickens N,O-carboxymethyl chitosan showed a low toxicity and adverse effect. Its clinical use is safe.更多还原