【摘要】 [目的]研究在高产奶牛日粮中添加膨化全脂大豆对乳脂中多不饱和脂肪酸(PUFA)含量的影响。[方法]选择胎次、体重相近,产犊50天左右的泌乳高峰期中国荷斯坦奶牛12头随机分为四组,采用4×4复合拉丁方试验设计,研究日粮中添加膨化全脂大豆对高产奶牛乳脂中PUFAs含量的影响。在TMR全混合日粮中加入不同比例(0、1kg、1.7kg、2.5kg)的膨化全脂大豆,每期试验21天,共四期。每期试验第15、17、19、21天取奶样。利用气相质谱联用技术(GC/MS)测定乳脂中PUFAs的相对含量。[结果]试验结果表明,膨化全脂大豆添加组与对照组相比,长链不饱和脂肪酸的相对含量显著增加(P<0.05),中短链饱和脂肪酸的相对含量下降极显著(P<0.01)。膨化全脂大豆各添加组间相比,1.7kg膨化全脂大豆添加水平下C18:2相对含量高于2.5kg膨化全脂大豆的添加水平,但差异不显著(P>0.05);C18:1t9在添加量为1.7kg水平下分别与1kg、2.5kg添加量相比有显著差异(P<0.05)。[结论]在高产泌乳奶牛日粮添加1.7kg膨化全脂大豆能够取得最佳的牛乳PUFA含量。更多还原

【Abstract】 [OBJECTIVE]A trial was conducted to evaluate the effect of dietary added extruded full-fat soybean on the content of PUFA of Milk Fat of Milking Cows. [METHOD]Twelve dairy cow with an average initial milking production of 26kg were randomly divided into four groups(adding 0,1,1.5,2.5kg EFFSB to the basal TMR respectively) according to replicated 4×4 Latin square design.The experimental time was 21 days every term. [RESULTS]The results showed that(1)Dietary supplementation of EFFSB increased the content of PUFA comparing the 0kg groups(P < 0.05) and decreased the content of middle and short FA significantly(P < 0.01).(2)Comparing with the 2.5kg groups,the content of C18:2 is higher for 1.7kg groups,but the differences was not significant(P > 0.05).(3)The content of C18:1t9 increased significantly comparing 1kg groups and 2.5kg groups(P < 0.05). [CONCLUSION]According to the content of PUFA and CLA in the milk,the optimum adding quantity of EFFSB in the dairy cow ration was 1.7kg.更多还原