【摘要】 以桂花品种金狮桂O.fragrans cv.Jinshi为材料,采取幼年型材料中下部饱满的腋芽茎段或成年型顶端的芽茎段作为外植体诱导丛生芽.结果表明:先用75%酒精消毒20 s,再用0.1%升汞消毒1.5 min,褐化率与污染率都较低,丛生芽的诱导率较高;金狮桂丛生芽的最佳诱导培养基为1/2MS+NAA 0.2 mg.L-1+6-BA0.5 mg.L-1+30 g蔗糖;试验中发现加入PVP虽然可以降低桂花褐化率,但同时抑制了丛生芽的生长;在丛生芽的增殖培养中,添加0.1~0.5 mg.L-1的6-BA与0.05 mg.L-1的NAA,同时添加1.00 mg.L-1的GA3丛生芽增值效果更佳.更多还原

【Abstract】 By adopting the stem section of the lower-part energetic axillary bud or the stem section of the top adult bud of young O.fragrans cv.Jinshi as explants,its tissue culture was made to induce axillary buds.The time of 75% alcohol disinfection is 20 seconds and the time of 0.1% HgCl is 1.5 minutes,which reduces the polluting rate and melting brown rate and improves the inducing rate of the bud.The optimal initial media is 1/2MS+NAA 0.2 mg·L-1+6-BA 0.5 mg·L-1 +saccharose 30 g.PVP added to the culture can reduce the brown rate but inhibit the growth of the bud.During the hyperplasia culture for axillary buds,adding 0.1～0.5 mg·L-1 6-BA and 0.05 mg·L-1 NAA and 1.00 mg·L-1 GA3 to the culture medium can increase the hyperplasia rate.更多还原