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牛乳中β-内酰胺类抗生素残留检测方法的对比研究

Evaluation of two microbial standard for detecting β-lactams in milk

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【作者】 李延华王伟君张兰威

【Author】 LI Yan-hua1,WANG Wei-jun1,ZHANG Lan-wei2,(1.College of Food Science,Northeast Agriculture University,Harbin 150030,China;2.Research Institute of Food Science and Genetics Engineering,Harbin Industry University,Harbin 150086,China)

【机构】 通化师范学院制药与食品系哈尔滨工业大学食品科学学院 吉林134002吉林134002黑龙江哈尔滨150086

【摘要】 对乳中抗生素残留的2种微生物检测法进行了比较,确定2种方法对6种广谱-β内酰胺类抗生素的检测限,同时指出了国标TTC法对6种广谱抗生素均不能检出欧盟法典规定的MRL残留浓度,而AOAO纸片法对其检测限均在MRL浓度范围内,且符合欧盟药典确定的最大残留量限制。建议乳中抗生素残留检测过程中,采用特异性与灵敏度高、经济简便、检测限达到MRLs规定的AOAO纸片法作为国标TTC检测法的补充。

【Abstract】 Two microbial standard methods to detect β-lactam residues in milk were compared in this paper.The detection limits of two methods for six kinds of β-lactams were determined.The results showed that the TTC method could not detect the MRL antibiotic residual concentration regulated by EU,while the AOAC paper disk method could.The AOAC method was suggested to be a supplement of TTC method to determine residual β-lactams in milk because of its specificity,sensitivity,convenience and economy.The detection limit of AOAC method for the six β-lactams also measured up the MRLS regulation.

  • 【分类号】S859.84
  • 【被引频次】3
  • 【下载频次】260
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