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夹层杯集菌离心涂片法检测痰抗酸杆菌对肺结核诊断价值的研究

The value of detection mycobacterium tuberculosis by centrifuging the liquored sputa and collecting bacteria in diagnosis of pulmonary tuberculosis

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【作者】 王改曹青山王金绳柴青峰高建彬张斌来中海尚好珍

【Author】 WANG Gai,CAO Qing-shan,WANG Jin-sheng,et al(Department of TB Mediicine.The First Affiviated Hospitial of Xinxiang Medical College,Weihui 453100,China)

【机构】 新乡医学院第一附属医院暨河南省结核病医院新乡医学院第一附属医院暨河南省结核病医院 河南卫辉453100河南卫辉453100

【摘要】 目的:探讨夹层杯集菌离心涂片法(夹层杯法)检测痰抗酸杆菌对肺结核的诊断价值。方法:采用夹层杯法使痰液充分液化,利于抗酸杆菌充分集中沉淀于特制的夹层杯底部的菌膜上,从而提高检出率。结果:该方法检测抗酸杆菌的阳性率为31.6%,分别明显高于本研究所传统涂片法的15.9%,培养法的11.9%,分别具有非常显著的临床意义(P<0.005)。结论:该方法简单、省时、阳性率高,并且便于进行质量控制,可能是一种具有推广价值的检测方法。

【Abstract】 Objective:Now positive outcome in sputa swear and cutural has strong diagnosis value in pulmonary tuberculosis,but the positive rate is low,the time of sputa cutural is long,finding out a new sputa checking way to improve the diagnosis of pulmonary tuberculosis.Methods:The testing box,Peng’s bug and QT-12 automatic centrifuge were provided by TianQi-Tech new medical systems co,LTD.The sputa were liquored completely and high-speed centrifuged in order to make the Mycobacterium tuberculosis sink and collect on a special membrane,detecting the bacteria on the membrane,the positive results were obviously improved by the way.Results:The positive rate of this new way is 31.6%,it is significently higher than 15.9% of sputa swear and 11.9% of sputa cultural.There was a significently difference(P<0.005)in positive rate among them.Conclusion:The new checking way is simple,sparing time,high positive rate and speciality,easily queality control,can be used as a valuable detecting way of mycobacterium tuberculosis in sputa in clinical.

【关键词】 夹层杯抗酸杆菌诊断肺结核
【Key words】 Collecting bacteriaPulmonaryTuberculosis
  • 【文献出处】 中国民康医学 ,Medical Journal of Chinese People’s Health , 编辑部邮箱 ,2007年16期
  • 【分类号】R446.5;R521
  • 【被引频次】7
  • 【下载频次】54
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