节点文献
SLE患者外周血CD4~+CD25~+调节性T细胞及相关基因Foxp3的变化
The changes of CD4~+CD25~+ T cells and expression of Foxp3 mRNA in peripheral blood mononuclear cell from patients with systemic lupus erythematosus
【摘要】 目的研究系统性红斑狼疮(SLE)合并狼疮性肾炎患者经肾上腺糖皮质激素(简称激素)冲击治疗后,外周血CD4+CD25+调节性T细胞(regulatoryTcell,Treg)及相关基因Foxp3表达的变化,从而探讨CD4+CD25+Treg和Foxp3与SLE发病的相关性。方法采用流式细胞术检测SLE患者外周血单个核细胞(PBMC)中CD4+CD25+T、CD4+CD25highT细胞数量的变化,RT-PCR检测PBMC中CD4+CD25+Treg功能相关基因Foxp3mRNA的表达。结果激素冲击治疗后的SLE患者CD4+CD25+T/CD4+T及CD4+CD25highT/CD4+T比值高于正常对照组;Foxp3mRNA水平表达与对照组差异不显著。结论CD4+CD25+Treg数量和功能的变化可能参与SLE的发病,激素可能通过提升CD4+CD25+Treg治疗SLE。
【Abstract】 Objective To investigate if CD4+CD25+ regulator T cells (Treg) and transcription factor Foxp3 is related with systemic lupus erythematosus (SLE) by quantify CD4+CD25+ regulatory T cells in peripheral blood from SLE patients after treatment by high-dose glucocorticoids.Methods Flow cytometry was used to analyze the proportion of CD4+CD25+ T and CD4+CD25 highT cells in peripheral1blood mononuclear cells (PBMC). The expression of Foxp3 mRNA in PBMC was detected by RT-PCR.Results After treatment, the proportion of CD4+CD25+T/CD4+Tcells and CD4+CD25 highT /CD4+Tcells were significantly higher in SLE group than those in control group (P<0.05), but the expression of Foxp3 mRNA had no difference between SLE patients and controls (P>0.05).Conclusions The changes of CD4+CD25+ Treg cell in number and function might participate in onset of SLE. The glucocorticoid may increase CD4+CD25+Treg frequency to treat SLE.
【Key words】 CD4+CD25+ T cell; Foxp3; Systemic lupus erythematosus (SLE);
- 【文献出处】 中国老年学杂志 ,Chinese Journal of Gerontology , 编辑部邮箱 ,2007年02期
- 【分类号】R593.241
- 【被引频次】16
- 【下载频次】494