【摘要】 目的探讨黄曲霉毒素G1长期灌胃对NIH小鼠肺泡上皮细胞SP-C和PCNA蛋白表达的影响。方法将60只NIH小鼠随机分为3组:AFG1小剂量组(3μg/kg组)、AFG1大剂量组30μg/kg组和对照组。实验组经口灌胃NIH小鼠AFG1,对照组灌胃同等容量生理盐水,3次/周,连续灌胃24周。实验第58周处死实验动物,光镜下观察肺组织病理学改变,采用免疫组化染色方法检测Ⅱ型肺泡上皮细胞特异分化标志物SP-C蛋白和细胞增殖活性相关抗原PCNA的表达情况。结果小剂量AFG1组和大剂量AFG1组肺泡上皮细胞SP-C标记指数分别为(31.63±5.51)%和(33.58±4.84)%明显高于对照组(19.72±1.92)%,P<0.01。小剂量AFG1组和大剂量AFG1组肺组织PCNA标记指数分别为(27.64±9.22)%和(30.08±9.35)%也明显高于对照组(7.91±1.89)%,P<0.01。结论黄曲霉毒素G1长期灌胃NIH小鼠可以促进肺泡上皮细胞中Ⅱ型肺泡上皮细胞特异分化标志物SP-C和细胞增殖活性相关抗原PCNA的表达。更多还原

【Abstract】 Objective To explore the effects of oral administration of aflatoxin G1 on SP-C and PCNA expression of alveolar epithelial cells in NIH mice.Methods NIH mice were randomly divided into three groups. Two experimental groups were treated intragastrically by gavage with AFG1 3g/kg and AFG1 30g/kg respectively, 3 times a week for 24 weeks. The mice in control group were correspondently treated with normal saline. All mice were fed with food that was free of AFGs as confirmed by HPLC analysis and then sacrificed and examined pathologically at the 58 weeks. The expression of SP-C and PCNA alveolar epithelial cells were determined by immunohistochemical staining.Results The immunohistochemical labelling index of SP-C of alveolar epithelial cells in AFG1 3g/kg and AFG1 30g/kg group were (31.63±5.51)% and (33.58±4.84)% respectively, which were significantly higher than that in control group(19.72±1.92)%,P<0.01. The labelling index of PCNA of alveolar epithelial cells in AFG1 3μg/kg and AFG1 30μg/kg group were significantly higher than that in control group (27.64±9.22)% and (30.08±9.35)% vs (7.91±1.89)%,P<0.01.Conclusion Oral administration of AFG1 can significantly increase the expression of SP-C and PCNA of alveolar epithelial cells in NIH mice.更多还原