【摘要】 目的探讨极低频率电磁场对体外培养肿瘤细胞的抑制作用。方法将人成骨肉瘤细胞(MG63)、乳腺癌细胞(MCF7)和肝癌细胞(HepG2)接种到96孔板中,并置于磁场强度分别为10mT、20mT、40mT、60mT的磁场中进行干预,每天刺激2次,每次1h,作用3天,同时每种细胞设置正常条件下的对照组。3天后以MTT法检测细胞活性(OD值),并采用流式细胞技术(AnnexinV—FITC和PI双标)检测3种细胞的凋亡情况。结果72h后以MTT法检测3种细胞的OD值,并于正常对照组进行比较,SPSS软件统计结果显示,磁场组的OD值较正常组低(P<0.05);流式细胞技术结果显示,磁场组肿瘤细胞凋亡细胞的阳性比例高于正常组,两组之间的差异有显著性统计学意义。结论极低频率电磁场对肿瘤细胞有明显抑制作用,在一定程度上诱导肿瘤细胞的凋亡。更多还原

【Abstract】 Objective To study effects of extremely low frequency electromagnetic field (EMF) on apoptosis of tumor cells in vitro. Methods Put human osteogenic sarcoma cells(MG63)、breast cancer cells(MCF7)、liver cancer cells(HepG2) in 96-hole plates. These plates were exposed to electromagnetic field(10mT、20mT、40mT、60mT) for stimulation twice per day lasting 1 hour for 3 days. The sham-exposure controls were correspondingly established. Inverted phase-contrast microscope was used to observe the cultured cells. MTT assay were applied to reveal viable cells with the OD value, and flow cytometry was used to detect the apoptosis results with Annexin V-FITC and PI.Results We compared the electromagnetic field groups with the normal groups by MTT assay after 72 hours. Analyzing the data in SPSS statistical software, we found the OD value of electromagnetic field groups were significantly less than the control groups (P< 0.05 ). The rates of apoptosis cells by flow cytometry revealed EMF groups were higher than that in control groups. The contrast of two groups was significant. Conclusion Extremely low frequency electromagnetic field conspicuously restrained the growth status of tumor cells, and some tumor cells could be induced to apoptosis by EMF.更多还原