【摘要】 目的:探讨人参皂甙(ginsenosides,GS)对睡眠剥夺(sleep deprivation,SD)大鼠学习记忆和活动性的影响。方法:用小平台水环境法建立SD模型。选用Sprague-Dawley大鼠,根据SD时间的不同(0～96h),随机分为5组,每组又分为实验组(E)和对照组(C),实验组给予GS,观察各组大鼠SD前后“Y”迷宫、跳台和旷场实验中的行为变化。结果:①“Y”迷宫实验:24h、48h、72h和96h的正确反应率,E显著高于C(P<0.05),48h、72h和96h的触电时间,E显著低于C(P<0.05),72h差别最为明显(P<0.01);②跳台实验:48h、72h和96h的第一次触电潜伏期,E显著高于C(P<0.05),触电时间,E显著低于C(P<0.05),72h和96h的触电次数,E显著低于C(P<0.05),72h的差别最为显著(P<0.01)。③旷场实验:72h和96h的中央格停留时间,E显著低于C(P<0.05);48h和72h的水平活动得分和垂直活动得分,E显著高于C(P<0.05),72h差别最为明显(P<0.01)。结论:GS对SD造成的大鼠学习记忆和活动性的损害有明显保护作用。在一定SD时间内,这种保护作用随SD时间的延长而增强。更多还原

【Abstract】 Objective: To explore the effects of ginsenosides(GS) on learning,memory and activity of rats during sleep deprivation(SD).Methods: SD was induced in Sprague-Dawlay rats by employing "flower pot" technique.The rats were randomly divided into five groups according to the time of SD(0-96h),and every group was also divided into two subgroups: experimental group(E) and control group(C).Forced feeding of GS to the experimental rats for 5 d before the rats were deprived of sleep.Before and after SD immediately,the behaviors of rats were studied in "Y" maze test,step-down test and open-field test.Results: ①"Y" maze test: As compared with those of the controls,the accuracy of response of experimental groups increased significantly in 24h,48h,72h and 96h(P<0.05),and the time of shock decreased markedly in 48h,72h and 96h(P<0.05).The difference reached the climax in 72h(P<0.01).②Step-down test: As compared with those of the controls,the latency of first shock of experimental groups increased significantly in 48h,72h and 96h(P<0.05),and the time of shock decreased markedly in 48h,72h and 96h(P<0.05);The difference reached the climax in 72h(P<0.01).③Open-field test: As compared with those of the controls,the time of centering of experimental groups increased significantly in 72h and 96h(P<0.05),and the crossing score and rearing score increased significantly in 48h and 72h(P<0.05).The difference reached the climax in 72h(P<0.01).Conclusion: GS can improve the rat’s impairment of learning,memory and activity induced by SD,and the effect may enhance with the increase of SD time to a certain extent.更多还原