【摘要】 研究比浊法测定硫酸多黏菌素E效价的影响因素和规律,建立了硫酸多黏菌素E的比浊法快速测定方法,检测时间由杯碟法的20h缩短到4h。当硫酸多黏菌素E的效价为30~100u/ml时,检测菌液接种浓度为5%,培养时间为4h,吸光度对数值与抗生素效价具有良好的线性关系。本法重现性好,相对误差小于5%。更多还原

【Abstract】 A turbidimetry assay for fast determination of polymyxin E potency was developed by studying on the influencing factors.Compared with Oxford assay,turbidimetry assay decreased the determination time from 20 hours to 4 hours.There was a linear relationship between the potency of polymyxin E and the logarithm of absorbency in the range of 30～100u/ml and inoculating concentration was 5%,with 4-hour culture time.The turbidimetry assay had a good reproducibility,and the relative error was less than 5%.更多还原