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比浊法快速测定硫酸多黏菌素E效价

Fast determination of the potency of polymyxin E by turbidimetry assay

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【作者】 佟斌吴兆亮殷昊赵艳丽

【Author】 Tong Bin,Wu Zhao-liang,Yin Hao and Zhao Yan-li(Department of Bioengineering,Hebei University of Technology,Tianjin 300130)

【机构】 河北工业大学生物工程系河北工业大学生物工程系 天津300130天津300130

【摘要】 研究比浊法测定硫酸多黏菌素E效价的影响因素和规律,建立了硫酸多黏菌素E的比浊法快速测定方法,检测时间由杯碟法的20h缩短到4h。当硫酸多黏菌素E的效价为30~100u/ml时,检测菌液接种浓度为5%,培养时间为4h,吸光度对数值与抗生素效价具有良好的线性关系。本法重现性好,相对误差小于5%。

【Abstract】 A turbidimetry assay for fast determination of polymyxin E potency was developed by studying on the influencing factors.Compared with Oxford assay,turbidimetry assay decreased the determination time from 20 hours to 4 hours.There was a linear relationship between the potency of polymyxin E and the logarithm of absorbency in the range of 30~100u/ml and inoculating concentration was 5%,with 4-hour culture time.The turbidimetry assay had a good reproducibility,and the relative error was less than 5%.

【关键词】 硫酸多黏菌素E比浊法效价
【Key words】 Polymyxin ETurbidimetry assayPotency
  • 【文献出处】 中国抗生素杂志 ,Chinese Journal of Antibiotics , 编辑部邮箱 ,2007年05期
  • 【分类号】S859
  • 【被引频次】13
  • 【下载频次】422
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