【摘要】 目的分析鲍曼不动杆菌的耐药性及其产OXA-23型碳青霉烯酶基因的核苷酸序列。方法用自动微生物分析仪测定常用抗菌药的M IC50;对OXA-23型碳青霉烯酶基因进行PCR扩增,产物纯化测序。结果15株产ESBL s鲍曼不动杆菌中有12株携带OXA-23型碳青霉烯酶;PCR产物纯化后测序表明与鲍曼不动杆菌(AY 795964.1)blaOXA-23基因序列100%同源。结论携带OXA-23型碳青霉烯酶基因的鲍曼不动杆菌对临床常用抗菌药的耐药率高,其编码基因为blaOXA-23。更多还原

【Abstract】 Objective To analyze drug resistance of Acinetobacter baumannii and it′s OXA-23 carbapenemase-encoding gene.Methods PCR amplification was performed of OXA-23 carbapenemase-encoding gene plasmid by PCR,PCR products purifing and nucleotide sequencing.Results There were 12 OXA-23 carbapenemase-producing strains in 15 isolates of ESBLs(+) A.baumannii.Sequencing analysis of this product revealed 100% identity with blaOXA-23 which was originally detected in another strain of A.baumannii(AY795964.1).Conclusion An isolate of Acinetobacter baumannii producing OXA-23 carbarpenase was highly resistant to commonly use antibiotics and it′s encoding gene is blaOXA-23.更多还原