【摘要】 目的:比较雷洛昔芬与雌二醇对成骨细胞增殖分化及TGF-β表达的影响。方法:本实验在新生SD大鼠颅盖骨第二代成骨细胞培养液中加入不同浓度的雷洛昔芬,并设立雌二醇阳性对照组及空白血清对照组,对其细胞增殖、碱性磷酸酶(ALP)活性、Ⅰ型胶原(α2)链Col-ImRNA、转化生长因子(TGF-β1)mRNA的表达进行检测分析。结果:体外培养成骨细胞在加入不同浓度雷洛昔芬和雌二醇后,(1)细胞增殖率与空白对照组比较无统计学意义(P>0.05)。(2)ALP活性和Col-I mRNA表达与雷洛昔芬成剂量依赖性,并在高浓度组与对照组比较有统计学意义(P<0.01)。(3)TGF-β1 mRNA的表达在雷洛昔芬10-8mol/L浓度组与对照组比较有统计学意义(P<0.05)。雌二醇对ALP活性、Col-I mRNA表达及TGF-β1 mRNA表达与对照组相比较均有统计学意义。结论:雷洛昔芬与雌二醇对体外培养成骨细胞均可上调细胞分化及骨胶原表达,对细胞增殖影响均不显著,雌二醇显著上调TGF-β1mRNA表达,且与雷洛昔芬相比较有统计学意义。此结果提示雷洛昔芬在通过雌激素受体(ER)发挥拟雌激素作用时与雌二醇作用机制可能不同。更多还原

【Abstract】 Objective: To investigate the effect of raloxifene and estradiol on the proliferation,differentiation and the expression of transforming growth factor-β(TGF-β) of osteoblasts in vitro.Methods: Different doses of roloxifene and estradiol were added into the medium of the second generation osteoblasts from the skull of newborn SD rats.The cell proliferation,activity of alkaline phosphatase(ALP),the level of typeⅠcollagen(Col-I) mRNA and TGF-β1 mRNA in different groups were measured and analyzed. Results: Raloxifene and 17-βE2 had no significant effects on stimulating the proliferation of osteoblasts(P>0.05 vs the control).However,raloxifene can significantly improve the ALP activity and Col-I mRNA expression in high consistency group(P<0.01) in dose-dependent manner.Raloxifene group in 10-8 mol/L increased the expression of TGF-β1 mRNA(vs the control,P<0.05).Estradiol significantly increased the ALP activity Col-I mRNA expression and TGF-β1 mRNA expression(P<0.05 or P<0.01 vs the control). Conclusion: Both of raloxifene and estradiol could stimulate differentiation of osteoblasts and expression of bone matrix,but had no effects in proliferation of cultured osteoblasts.The expression of TGF-β1 mRNA were different,which implied their different mechanisms through estrogen receptor.更多还原