【摘要】 目的:研究环氧合酶-2(COX-2)在小鼠足细胞中的表达情况,为进一步研究嘌呤霉素氨基核苷(PA)诱导足细胞凋亡过程中特异性COX-2的表达,以及特异性COX-2抑制剂对足细胞的保护作用建立实验基础。方法:将体外培养的条件永恒的足细胞复苏并传代培养,采用Western blot法分析COX-2在足细胞中的表达。结果:在33℃增殖状态下,足细胞呈鹅卵石状,在37℃分化状态下的足细胞呈分叉状。正常足细胞在37℃分化14 d后,用低血清培养液培养后,随着时间延长,COX-2表达逐渐增多,24 h达最高,以后逐渐减少。结论:COX-2在足细胞上的表达呈时间依赖性。更多还原

【Abstract】 Objective: To investigate the expression of Cyclooxygenase-2 in cultured murine podocytes in order to study the COX-2 expression in apoptotic cells exposed to puromycin aminonucleoside.Methods: Experiments were performed using growth-restricted,conditionally immortalized mouse podocytes.Western blot was used to detect the expression of COX-2 in podocytes. Results: At the permissive temperature of 33℃,the conditionally immortalized human podocytes grew in a cobblestone morphology.They are grown arborized under nonpermissive conditions at 37℃.For passaging cells,podocytes were grown under growth restrictive conditions at 37℃ for 14 days.The COX-2 expression increased over time.The highest level was found at 24 hour.Afterwards it decreased gradually. Conclusion: The COX-2 expression in podocytes was time-dependent.更多还原