【摘要】 目的:研究高碳酸血症(HPC)对急性肺损伤(ALI)时肺泡Na+-K+-ATP酶活性的影响。方法:将24只SD大鼠随机分为3组,每组8只:正常对照组(Ⅰ组);ALI组(Ⅱ组);ALI+HPC组(Ⅲ组)。用0.2 mol/L盐酸(2 ml/kg)气管内滴入建立大鼠急性肺损伤模型,吸入8%CO2气体建立高碳酸血症模型。以肺组织病理变化、支气管肺泡灌洗液(BALF)细胞计数和蛋白浓度、肺湿干重比(W/D)、血和BALF中TNF-α浓度及IL-8浓度为评估肺损伤的指标;以Na+-K+-ATP酶活性变化为评估HPC疗效的指标。结果:Ⅲ组BALF细胞计数(1.02±0.48),W/D(7.24±0.58),BALF蛋白浓度(0.25±0.16)和IL-8浓度(29.95±7.11)比Ⅱ组相应指标(1.79±0.73;8.60±1.24;0.53±0.35;59.52±36.00)明显降低(P<0.01);Ⅲ组血TNF-α浓度(83.86±46.93)、IL-8浓度(80.00±24.72)比Ⅱ组相应指标(161.57±54.12;110.00±15.92)明显降低(P<0.01);Ⅲ组Na+-K+-ATP酶活性(13.23±1.20)比Ⅱ组(10.77±2.21)明显增高(P<0.01)。结论:HPC对ALI时肺泡Na+-K+-ATP酶的活性有保护作用,这可能是HPC对盐酸诱导的大鼠ALI保护作用的重要机制。更多还原

【Abstract】 Objective: To study the protective effect of hypercapnia(HPC) on the activity of Na+-K+-ATPase in rat acute lung injury(ALI) induced by hydrogen chloride(HCL).Methods: Twenty Sprague-Dawley rats were randomized into three groups: groupⅠ(normal control),groupⅡ(ALI) and groupⅢ(ALI+HPC).ALI is induced by HCL(0.2 mol/L) instillated into bronchial(2 ml/kg).HPC is induced by inspiration of 8% CO2.The changes of lung histology,lung wet-to-dry weight ratios,bronchial alveolar lavage fluid(BALF) cells count,serum and/or BALF concentration of TNF-α,IL-8 and the activity of Na+-K+-ATPase were used to assess injury and the effects of PHC. Results: The indices of lung inflammation including BALF cell count(1.79±0.73),the concentration of IL-8(59.52±36.00) and protein(0.53±0.35)in BALF,W/D ratios(8.60±1.24) were higher in groupⅡ than those in group Ⅲ(P<0.05).The serum concenuration of TNF-α(161.57±54.12),IL-8(110.00±15.92) were higher in groupⅡ than those in group Ⅲ(P<0.01).The activity of Na+-K+-ATPase(10.77±2.21)in lung tissue homogenate in group Ⅱ was lower than that in group Ⅲ(P<0.01). Conclusion: HPC had protective effect on the activity of Na+-K+-ATPase in rat ALI-induced by HCL.This effect may be one of the mechanisms of the protective effects of HPC on ALI.更多还原