【摘要】 目的:比较未修饰纳米SiO2和表面修饰型纳米SiO2对大鼠肺泡巨噬细胞的毒性。方法:通过支气管肺泡灌洗分离雄性SD大鼠肺泡巨噬细胞。未修饰纳米SiO2(M-5型)及两种表面修饰型纳米SiO2(TS-610型,TS-720型)剂量分别为0,8,40,100,200μg/cm2,用MTT实验测定对细胞活性的影响,LDH实验测定对细胞膜的损伤,TBA法测定脂质过氧化作用的程度。结果:M-5型纳米SiO2在剂量大于100μg/cm2时,各指标与对照组相比有统计学意义(P<0.05),而两种表面修饰型纳米SiO2与对照组相比均无统计学意义。结论:未修饰纳米SiO2对大鼠肺泡巨噬细胞的毒性较强,颗粒表面被修饰后其毒性明显降低,表明颗粒表面反应性在毒性机制中发挥重要作用。更多还原

【Abstract】 Objective: To compare the cytotoxicity of nanosized silica and surface modified nanosized silica in rat alveolar macrophages.Methods: Male SD rat alveolar macrophages isolated by bronchoalveolar lavage were exposed to nanosized silica(M-5) and surface modified nanosized silica(TS-610,TS-720) at doses of 0,8,40,100,200 μg/cm2.The cell activity was tested by MTT assay,injury to membrane by LDH assay and lipid peroxidation by TBA method. Results: For the three indices M-5 nanosized silica had significant difference from control when doses were higher than 100 μg/cm2(P<0.05),whereas surface modified nanosized silica had no significant difference from control at all doses. Conclusion: M-5 nanosized silica was more toxic to alveolar macrophages.The apparent decrease of cytotoxicity after surface modification indicated the important role of surface reactivity in the cytotoxicity of nanosized silica.更多还原