【摘要】 目的:探讨烫伤修复创面过程中MMP-2表达的意义及其人工合成抑制剂的作用。方法:将16只大鼠随机分成实验组和对照组,在每只鼠背部制成20%TBSA深二度烧伤模型,实验组每天食物中添加卡托普利1mg/kg,烫伤后分别在24 h,4 d,7 d,14 d,20 d,29 d,35 d,62 d切取背部创面标本,采用免疫组化方法检测标本中MMP-2的表达情况;在实验中取2只正常大鼠皮肤标本,同样检测其标本中MMP-2的表达情况。结果:MMP-2在正常大鼠皮肤中无表达;在大鼠烫伤后不同时期表达强度不同,24小时后开始表达,以后逐渐增强,大约到第20 d达到高峰,持续约15 d后减弱;但到创面愈合后仍有表达。实验组MMP-2表达水平明显低于对照组,大鼠烫伤后7,14,20,29 d实验组与对照组比较差异有显著性意义(P<0.05),并且实验组创面愈合时间早于对照组。结论:MMP-2表达水平在创面愈合过程中有一定规律,与烫伤创面的愈合和瘢痕的形成有密切关系;人工合成抑制剂可抑制其活性,从而促进创面愈合。更多还原

【Abstract】 Objective: To investigate the expression of MMP-2 in the process of scald wound repair and the role of its synthesized inhibitor.Methods: Sixteen big mice were randomly divided into two groups and their backs were scalded into 20% TBSA deep partial thickness burn.The experimental group was given food which contained Captopril 0.5~1mg/kg/day.Biopsies from wound skin harvested at 24PBHS and 4,7,14,20,29,35,62PBDS.The expression of MMP-2 in the samples were detected immunohistochemically.MMP-2 was also detected in the skin of another 2 normal mice as normal control. Results: MMP-2 was not expressed in the normal skins of mouse.After mice were scalded,MMP-2 had different expression in the process of scald wound repair.MMP-2 began to express at 24 PBHS,and enhanced gradually,and peaked on 20PBDS,and continued for 15days then reduced;but MMP-2 was still expressed up to wound healing.The expression of MMP-2 in experimental group was apparently lower than that of control group,which was significantly lower than that in control group at 7,14,20,29PBDS(P<0.05),and the healing time of wound in experimental group was earlier than that of control group. Conclusion: The expression level of MMP-2 had certain law during wound repair,the expression of MMP-2 was closely related to wound repair and the process of scar tissue growing;its synthesized inhibitor may inhibit its activity and accelerate wound healing.更多还原