【摘要】 目的:克隆人GITR基因全长编码区的cDNA,同时对其序列进行分析。方法:采用RT-PCR方法,从正常人外周血单个核细胞获得GITR基因的cDNA,克隆至pGEM-T载体,选择阳性克隆并进行序列测定。结果:扩增得到的人GITR基因编码区cDNA的全长726 bp,编码241个氨基酸残基,与GeneBank注册的序列完全一致。结论:获得人类GITR基因的克隆,为进一步研究其生物学功能奠定了基础。更多还原

【Abstract】 Objective: To clone and analyze a full-length cDNA encoding human glucocorticoid-inducible tumor necrosis factor receptor(GITR) gene.Methods: The cDNA of GITR was amplified by RT-PCR using the total RNA extracted from normal human peripheral blood mononuclear cell(PBMC).The PCR product was inserted into pGEM-T vector and then transformed into E.coli DH5α.The positive recombinant clone was analyzed by digestion of restriction endonuclease and DNA sequencing. Results: The cDNA of human GITR had a complete open reading frame with a length of 726 bp,which encoded a product of 241 amino acid,and shared 100% homology with the sequence of mRNA for GITR in Genbank. Conclusion: The cDNA of hGITR was cloned successfully,which posed a basis for further researching on its biological function.更多还原