【摘要】 从感染根结线虫的番茄根系中分离到46个细菌菌株,NA平板初筛获得13个抑制青枯雷尔氏菌生长的拮抗菌株,其抑菌圈宽度达7.1~26.3mm.温室试验表明,菌株EN5、EN15、EN01和EN16对青枯病的防治效果最为显著,平均防效分别为70.82%、89.54%、85.38%和78.81%;而EN5、SW1和SB2能有效减少番茄根结线虫病的根结指数,防效均达49%.同时测定这些菌株对青枯病菌-根结线虫复合病的防治效果,结果表明,EN5、SW1和SB2均能显著降低病情指数,其中SW1的防效最佳,青枯病指数比对照下降56.66,根结指数降低5.21.通过形态、生理生化特征和16SrDNA序列分析,初步鉴定菌株EN5、SW1和SB2为枯草芽孢杆菌(Bacillus subtilis).更多还原

【Abstract】 Forty-six bacterial strains were isolated from root-knot nematode infected tomato roots, 13 of which could inhibit the growth of Ralstonia solanacearum in vitro with the inhibition zone diameter ranging from 7.1 mm to 26.3 mm on NA plate. In the greenhouse tests, tomato bacterial wilt caused by Ralstonia solanacearum was controlled effectively by the isolates EN5, EN15, EN01 and EN16 with the average control efficacy of 70.82%,89.54%,85.38% and 78.81%, respectively. Root-knot disease caused by Meloidogyne incognita was reduced 49% by EN5, SW1 and SB2. They were also tested for their ability to control the complex disease caused by two pathogens, likewise, the isolates EN5, SW1 and SB2 significantly reduced the infection percentage compared with non-treated control, among which, SW1 showed best effects on reduction of incidence of bacterial wilt disease by 56.66 and root galling index by 5.21. Based on their physiological and biochemical characteristics and 16S rDNA sequences, EN5, SW1 and SB2 were identified as Bacillus subtilis.更多还原