【摘要】 铁皮石斛Dendrobium officinale是一种名贵药用植物,野生资源亟待保护,必须通过试管苗快繁技术研究,才能满足人们对它的需要。通过不同基本培养基对铁皮石斛进行种子发芽试验,不同消毒方法对茎尖进行存活率试验,不同植物生长调节物质组合对不定芽、原球茎和壮苗及根系的诱导试验的结果表明:铁皮石斛胚培养最佳培养基为1/2 MS(Murashigeand Skoog)+添加物A+25 g.L-1蔗糖;4种消毒方法中最佳消毒方法为B,外植物茎尖存活率可达76.2%。不定芽诱导最佳培养基为1/2 MS+1.5 mg.L-16-苄基腺嘌呤(BA)+0.25 mg.L-1萘乙酸(NAA),诱导倍数4.7。原球茎诱导最佳培养基为1/2 MS+1.0 mg.L-1BA+0.5mg.L-1NAA,诱导率为58%。不定芽最佳生根培养基为1/2 MS+0.5 mg.L-1NAA,生根率为100%,平均生根数为7.1条。建立了铁皮石斛试管苗快繁体系,为其广泛应用提供了依据。图1表5参11更多还原

【Abstract】 Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use.Its present resource cannot satisfy the needs.Tissue culture is a quick and convenient path to produce more iron-sheet dendrobium.The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog),half of MS(1/2 MS),Hyponexl and Hyponex 2.And the fresh stems were sterilized in different ways,then cultured in the basic media added NAA or BA in different ammounts.The resluts showed that 1/2 MS+A(the additive substance)+25 g·L-1 sugar was the best medium for seeding;Method B was the best disinfection with the livability 76.2%;1/2 MS+1.5 mg·L-1 BA+0.25 mg·L-1 NAA was better for adventitious buds induction,with 4.7 buds on each explant;1/2 MS+1.0 mg·L-1BA+0.5 mg·L-1NAA was fine for protocorm induction,with the rate of 8%;1/2 MS+0.5 mg·L-1 NAA was better for rooting,with a 100% rate and 7.1 roots on each explants.The proliferation systerm can enlarge the application of Dendrobium officinale.[Ch,1 fig.5 tab.11 ref.]更多还原