【摘要】 目的腹主动脉缩窄法(AO)建立大鼠慢性心力衰竭模型,研究B型钠尿肽(BNP)在评价大鼠心功能中的作用。方法Wistar大鼠80只,随机分为手术组和假手术组,术后第4、8、12和16周时行血流动力学监测,留取血清和病理标本,比较血流动力学指标、病理和血清学指标。结果与假手术组相比,大鼠AO术后4周,BNP水平显著升高(P<0.05),并在观察期间进行性升高,心肌细胞直径(CMD)、心脏质量指数升高。术后8周-dp/dtmax降低。术后12周LVSP升高,+dp/dtmax降低。术后16周上述改变更明显。BNP水平与血流动力学和心肌肥厚指标相关性良好。结论腹主动脉缩窄可成功复制大鼠慢性心力衰竭模型,BNP对模型大鼠心功能受损高度敏感。更多还原

【Abstract】 Objective To establish rat model of chronic heart failure by partially banding abdominal aorta for different time limits and to specify the effect of exercise test on evaluation of cardiac function of the model.Methods Eighty Wistar rats were randomly divided into two groups:operation group and sham operation group(each containing 40 rats).Cardiac mass index,hemodynamic parameters and cardiac morphological changes were investigated in different groups.Results Cardiac mass and cardiac mass index were increased from 4 weeks after aortic banding(P>0.05)compared with sham operation group,and more significant higher in 8 weeks after operation(P<0.05).BNP level,cardiac myocardial diameter(CMD)was increased compared with sham operation group from 4 weeks after aortic banding(P<0.001).The diastolic parameter of-dp/dtmax was lower than sham operation group significantly in 8 weeks after operation(P<0.001).LV systolic pressure(LVSP)was elevated and +dp/dtmax decreased after 12 weeks of aortic banding operation(P<0.05)compared with sham operation group,which were more significant in 16 weeks.There has a linear relationship between BNP level and hemodynamic parameters and cardiac morphological changes.Conclusion Abdominal aortic banding can successfully copy chronic heart failure model in rats.BNP level was highly sensitive to the damage of heart function.更多还原