【摘要】 目的探讨VEGF与下调c-myc联合干预对血管成形术后再狭窄的影响。方法中国大耳白兔28只,分为4组,每组7只；髂总动脉球囊拉伤后,分别给予反义c-myc和(或) VEGF,取病理切片对再狭窄程度进行评价；采取免疫组化及Western blotting对联合应用VEGF和反义c-myc效果进行评价。结果下调c-myc与VEGF均能减轻血管成形术后再狭窄,反义c-myc与VEGF联合应用则显著减轻再狭窄,但两者间无显著交互作用(P＞0．05)。结论联合干预c-myc与VEGF显著减轻再狭窄,但两者之间无协同作用。更多还原

【Abstract】 Objective To investigate the effect of downregulation c-myc and VECF in combinat- ed applying on restenosis after angioplasty.Methods 28 China rabbits were divided randomly into four groups with each group containing 7 rabbits.After ball-injured,antisense c-myc or/and VEGF was performed.Group A as control,group B antisense c-myc respectively,group C VEGF only,group D an- tisense c-myc and VEGF.Evaluating on restenosis by pathological section,immunohistochemistry and Western blotting’for the expressing of PCNA.Results Pathological section:(intimal and media)both downregulation c-myc and VEGF can relieve restenosis after angioplasty,combining antisense c-myc with VEGF can relieve restenosis more effectly.The situations of immunohistochemistry and Western blot- ting were on the analogy of pathological section.Conclusions Downregulation c-myc and VEGF has definite effect in preventing restenosis after angioplasty.Combining antisense c-myc with VEGF do not a- chieve concerted effect.更多还原