【摘要】 目的了解大肠埃希菌(ECO)新的氨基糖苷类修饰酶基因存在状况。方法用聚合酶链反应(PCR)法对34株分离自患者的ECO进行aac(6′)-Ⅰb、aac(6′)-Ⅱ、aac(3)-Ⅰ、aac(3)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ等6种氨基糖苷类修饰酶基因检测研究。结果34株ECO中氨基糖苷类修饰酶基因aac(6′)-Ⅰb阳性8株(23.5%)、aac(3)-Ⅱ阳性27株(39.4%)、ant(3″)-Ⅰ阳性3株(8.8%),其余基因均阴性。结论氨基糖苷类高耐药性与氨基糖苷类修饰酶基因高检出率(85.3%)有关;在同一所医院的ECO中同时检出aac(6′)-Ⅰb和aac(6′)-Ⅰb-cr两种亚型,为国内外首次报道。更多还原

【Abstract】 OBJECTIVE To detect novel resistance genes encoded aminoglycoside-modifying enzymes from clinical isolates of Escherichia coli.METHODS All isolates were subjected to polymerase chain reaction(PCR) assays targeting aminoglycoside-modifying enzymes genes including aac(6′)-Ⅰb、aac(6′)-Ⅱ,aac(3)-Ⅰ,aac(3)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ.RESULTS In thirty-four strains of E.coli,aac(6′)-Ⅰb were detected in 8(23.5%),aac(3)-Ⅱ in 27(39.4%),ant(3″)-Ⅰ in 3(8.8%),and others were negative.CONCLUSIONS This study showed that high resistance to aminoglycosides was related to high positive rate(85.3%) of aminoglycoside-modifying enzymes genes in E.coli;and it was also first reported that both aac(6′)-Ⅰb and aac(6′)-Ⅰb-cr were detected in clinical isolates of E.coli only from a hospital.更多还原