【摘要】 目的探讨乙型肝炎病毒表面大蛋白(LHBs)用于临床乙型肝炎诊断的实验价值及与乙型肝炎病毒DNA定量的相关性。方法应用基因工程表达的乙型肝炎病毒表面大蛋白,采用ELISA技术共检测了510份HBsAg阳性血清标本中的LHBs,同时检测前S1抗原(PreS1Ag)及HBeAg,并应用荧光定量PCR技术平行检测HBVDNA。结果380份HBV DNA阳性血清标本中,361份(95.00%)LHBs检测阳性,PreS1Ag192份(50.50%)检测阳性;LHBs阳性率明显高于乙型肝炎前S1阳性率,两者差异有统计学意义(P<0.01);239份HBeAg阴性血清标本中,HBV DNA129份(53.93%)阳性,LHBs132份(55.23%)阳性,PreS1Ag77份(32.22%)阳性,LHBs阳性率与HBV DNA阳性率差异无统计学意义(P>0.05),均显著高于PreS1Ag;LHBs含量与HBV DNA拷贝数呈正相关性,相关系数r=0.946。结论LHBs是一个操作简便的可用于反映乙型肝炎病毒复制水平的敏感指标。更多还原

【Abstract】 OBJECTIVE To review the clinical value of hepatitis B virus large surface protein(LHBs)used for diagnosis of the clinical hepatitis B patient and relativity with hepatitis B virus DNA.METHODS Genetic engineering technology was used to express the LHBs.Enzyme linked immunosorbent assay(ELISA) methods were used to examine the LHBs,PreS1Ag and HBeAg and quantitative real-time PCR methods were used to detect the HBV DNA of 510 HBsAg-positive serum samples.RESULTS ①Significant difference of positive rate was found between LHBs(95.0%,361/380) and PreS1Ag(50.50%,192/380) in 380 HBV DNA-positive serum samples.②No significant difference of positive rate was found between LHBs(55.23%,132/239) and HBV DNA(53.93%,129/239);significant difference of positive rate was found between LHBs(55.23%,132/239) and PreS1Ag(32.22%,77/239) in 239 HBeAg-negative serum samples.③The content of LHBs and the HBV DNA copies performed a nice positive relativity(r=0.946).CONCLUSIONS Our results demonstrate that the LHBs is a sensitive index in reflecting hepatitis B virus replication level and its operation is convenient.更多还原