【摘要】 目的研究铜绿假单胞菌对亚胺培南耐药机制。方法用PCR法对铜绿假单胞菌亚胺培南耐药相关的金属β-内酰胺酶IMP、VIM基因和外膜蛋白OprD2基因等3种主要耐药基因进行了检测与分析。结果35株耐亚胺培南铜绿假单胞菌IMP和VIM型金属酶基因检测均阴性,35株耐亚胺培南铜绿假单胞菌OprD2基因检测均阴性,但35株铜绿假单胞菌23S rRNA基因扩增阳性。结论研究表明湖北襄樊地区铜绿假单胞菌不产生金属酶,但从遗传学角度证实外膜蛋白OprD2基因缺失是襄樊铜绿假单胞菌对亚胺培南耐药的重要机制。更多还原

【Abstract】 OBJECTIVE To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.METHODS PCR method was used to detect and analyze P.aeruginosa imipenem-resistance associated IMP gene and VIM gene of metallo-β-lactamases and outer membrane protein D2（OprD₂） gene.RESULTS All 35（imipenem）-resistant P.aeruginosa strains were negative for IMP gene and VIM gene of metallo-β-lactamases;and for OprD₂ gene;but 23S rRNA gene of all 35 P.aeruginosa strains was positive.CONCLUSIONS The study suggested that in（Xiangfan） area,Hubei Province P.aeruginosa doesn′t produce metallo-β-（lactamases）,but in genetics it is（identified） that the loss of outermembrane protein D2（OprD₂） gene is the（essential） mechanisms of imipenem（resistance） in P.aeruginosa in Xiangfan area,Hubei Province.更多还原