【摘要】 研究了生物不对称催化苯乙酮酸合成(R)-(–)-扁桃酸的方法。通过对38株菌进行初筛,获得对底物苯乙酮酸具有较高转化活性的出发菌株假丝酵母Ca.3。进一步紫外与微波诱变,得到具有高转化率与高对映体选择性的突变菌株Ca.3.37.48。在转化培养中,选择初始底物浓度30mmol/L、pH7.0、温度32℃的条件,(R)-(–)-扁桃酸得率86.5%,对映体过量值为99.5%。更多还原

【Abstract】 Asymmetric biosynthesis of (R)-(–)-mandelic acid from phenylglyoxylic acid was investigated. Candida albicans (Ca.) 3 was selected as the original strain from 38 strains. Mutant Ca.3.37.48 with effective transforming ability and high enantioselectivity was obtained via ultraviolet irradiation and microwave mutagenesis. When it was incubated at 32℃, pH 7.0 in the presence of 30mmol/L substrate, the yield and the enantiomeric excess of (R)-(–)-mandelic acid could reach to 86.5% and 99.5%.更多还原