【摘要】 [目的]观察曲尼斯特(Tranilast)对放射性肺损伤大鼠的转化生长因子β1(TGF-β1)及Ⅰ、Ⅲ型胶原蛋白表达的影响。[方法]65只Wistar大鼠分为3组,曲尼斯特治疗照射组(实验组25只),单纯照射组(单照组25只),以及空白对照组(空白组15只)。照射后不同时间分别检测血浆TGF-β1含量,光镜下观察右肺组织形态学变化及免疫组化检测TGF-β1及Ⅰ、Ⅲ型胶原蛋白表达。[结果]实验组较单照组肺泡炎和纤维化出现晚且程度轻;照射后2个月TGF-β1表达比单照组降低(P=0.002);Ⅰ型胶原蛋白(从照射后3个月开始),Ⅲ型胶原蛋白(从照射后2个月开始)在实验组的表达明显低于单照组(P=0.001)。血浆TGF-β1检测显示,实验组的TGF-β1含量高峰延至照射后第3个月。[结论]曲尼斯特通过降低TGF-β1表达有效地减轻了放射性肺损伤;对减少Ⅰ、Ⅲ型胶原沉积有同等的作用,提示其潜在的抗肺纤维化治疗作用。更多还原

【Abstract】 [Purpose] To investigate the effect of Tranilast on expressions of TGF-β1,type Ⅰ and Ⅲ collagen induced by radiation lung injury in rats.[Methods] Sixty-five Wistar rats were divided into 3 groups:irradiation and Tranilast treatment group(experimental group),25 rats;irradiation alone group,25 rats;and control group,15 rats.Level of TGF-β1 in plasm was detected at different time points.Expression of TGF-β1,typeⅠand Ⅲ collagen was detected immunohistochemically.Morphological characteristics in right pulmonary were observed microscopically.[Results] Compared to irradiation alone group,the appearance of alveolitis and fibrosis was delayed and mild,and TGF-β1 expression was significantly lower in 2 months after irradiation in experimental group(P=0.002).TypeⅠcollagen(3 months after irradiation)and Ⅲ collagen deposition(2 months after irradiation)in experimental group significantly decreased compared with irradiation alone group(P=0.001).The peak of TGF-β1 in plasm was delayed until the third month after radiation in experimental group.[Conclusion] Tranilast alleviates radiation-induced lung injury via down-regulating TGF-β1 expression,as well as reducing biosynthesis of type I and Ⅲ collagens.It suggests that Tranilast is provided with therapeutic potential as an antifibrotic agent.更多还原