【摘要】 目的建立高效的围生期沙眼衣原体(Chlamydia trachomatis,CT)流行病学调查批处理方法--混合尿液(pooling urine)质粒缺口-连接酶链反应-酶联免疫吸附法(gap-ligase chain reaction-en-zyme linked immunoabsorbent assay,gap-LCR-ELISA)。方法比较质粒gap-LCR-ELISA对不同体积孕妇尿液混合库(4×,6×,8×,10×)及单份样本(1×)检出CT感染的灵敏度和特异度。结果质粒gap-LCR-ELISA检测4,6,8,10人份混合尿样中的CT感染均可达到100%的特异度,灵敏度分别为95%,85%,70%和65%,各组实验成本下降的比例均超过40%。结论4人份孕妇混合尿液质粒gap-LCR-ELISA的检测效能,等同于对单份标本的独立检测,为该法最适宜的标本混合策略,在大规模流行病学调查中具有应用价值。更多还原

【Abstract】 Objective To construct an effective pooling urine plasmid gap-LCR-ELISA method for large-scale epidemic screening of perinatal Chlamydia trachomatis(CT)infection.Methods The most optimal pooling size(4×,6×,8×,10×)was chosen by comparing the sensitivity,specificity and cost-effectiveness with single urine detection.Results Pooling urine by 4×,6×,8×,10× size to gap-LCR-ELISA resulted in a satisfying specificity up to 100%,but the sensitivity declined with the increase of pooling volume(95%,85%,70% and 65%,respectively).And a significant cost reduction of more than 40% was obtained in all the groups.Conclusion 4× size pooling urine strategy could achieve the same detecting capability as that of individual specimen examined by plasmid gap-LCR-ELISA,which is quite valuable in large scale epidemic screening for CT infection.更多还原