【摘要】 目的:利用RP-HPLC-ELSD测定不同产地的浙贝母类药材中的浙贝母碱、去氢浙贝母碱、浙贝宁的含量。方法:Xterra RP18色谱柱(3.9 mm×150 mm,5μm),流动相乙腈-10 mmol.L-1NH4HCO3(pH10.10),梯度洗脱,以ELSD检测。结果:浙贝母碱在0.12～24.0μg,去氢浙贝母碱在0.13～26.0μg,浙贝宁在0.16～31.0μg与峰面积的对数值呈线性关系;平均回收率分别为98.2%,98.2%,96.5%;RSD分别为1.3%,1.7%,2.2%。结论:本方法用于测定浙贝母药材中的主要生物碱浙贝母碱、去氢贝母碱、浙贝宁的含量简便、准确。更多还原

【Abstract】 Objective:To determine peimine,peiminine and zhebeinine in Fritillaria thunbergii simultaneously by RP-HPLC-ELSD.Method:The HPLC was carried out with a Waters Alliance,Model 2690,equipped with an Xterra TM RP18 column(3.9 mm×150 mm,5 μm),and evaporated light scattering detector.It was eluted gradiently with acetonitrile and 10 mmol·L-1 NH4HCO3(adjusted to pH 10.10 by ammonia solution).Result:The average recovery rates(n=5) of peimine,peiminine and zhebeinine were 98.2%(RSD 1.7%),98.2%(RSD 2.2%),96.5%(RSD 1.3%) respectively.Conclusion:The method is simple,sensitive and reliable.It can be used for quantitative determination of F.thunbergii.更多还原