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雷公藤属3种植物遗传关系与遗传多样性的RAPD分析

RAPD analysis for genetic relationship and diversity of three species of genus Tripterygium

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【作者】 刘万水郭宝林陈玉婷黄文华斯金平

【Author】 LIU Wan-shui1,GUO Bao-lin2,HUANG Wen-hua2,SI Jin-ping3(1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100094,China;2.Beijing University of Traditional Chinese Medicine,Beijing 100102,China;3.Zhejiang Forestry College Research Centre for Natural Pharmacology,Lin’an 323000,China;)

【机构】 北京中医药大学中药学院中国医学科学院中国协和医科大学药用植物研究所浙江林学院天然药物研究中心 北京100102北京100094北京100102浙江临安311300

【摘要】 目的:阐明雷公藤属植物的遗传关系和遗传多样性。方法:用RAPD分子标记方法研究来自雷公藤属植物3个种4个类型24个居群的110个样本。结果和讨论:从100个随机引物中筛选出10个引物,得到128条带,其中多样性条带123个,占96%。通过聚类分析可以看出,东北雷公藤明显与其他种有显著的遗传差异;中间类型和雷公藤的关系较与昆明山海棠的关系更密切,但中间类型和雷公藤之间也有明显的遗传分化,且中间类型呈现较雷公藤复杂的遗传多样性,不同的中间类型居群与雷公藤的关系远近不同。大量的中间类型的存在,连接了昆明山海棠与雷公藤这2个种,从分类上建议将2个种合并。将除去东北雷公藤的其他居群作为一个整体分析,居群间分化明显,遗传多样性主要存在于居群间,因此应当收集不同来源的种质以保存尽可能多的遗传多样性。

【Abstract】 Objective:To study the genetic relationship and diversity of 3 species which belong to Tripterygium.Method:Samples were collected and divided into 4 types:typical T.wilfordii,typical T.hypoglaucum and their middle type according to morphological characters,and T.regelii.RAPD markers were used to measure the genetic relationship and diversity of 110 individuals from 22 natural populations in China.Result and Conclusion:10 primers were selected from 100 ones screened.A total of 128 bands were scored and 123 of them were polymorphic.Cluster analysis indicated that all the samples could be divided into 3 parts:5 individuals from T.regelii gathered closely and were separated from other population and formed single branch.All 4 populations from T.hypoglaucum gathered directly.The middle type showed a nearer relationship with T. wilfordii than with T.hypoglaucum,although there was genetic differentiation between populations from middle type and those from T.wilfordii.The middle type has more diversity than T.wilfordii.The distance between different middle type populations and T.wilfordii was different.The existence of middle type populations between T.wilfordii and T.hypoglaucum suggested that the 2 species should be combined into one species.Considering all populations except T.regeli,the generic differentiation among populations was significant and the genetic diversity existed mainly among different populations. So samples of Tripterygii should be collected from different ppopulations for the reservation of the genetic diversity.

【基金】 浙江省科技厅科技计划项目(2003C32098)
  • 【文献出处】 中国中药杂志 ,China Journal of Chinese Materia Medica , 编辑部邮箱 ,2007年16期
  • 【分类号】S567.19
  • 【被引频次】29
  • 【下载频次】363
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