【摘要】 目的:观察地龙汤对实验性支气管哮喘豚鼠气道炎症的作用,并探讨其作用机制。方法:豚鼠48只随机分为正常组,模型组,地塞米松组,小青龙汤组,地龙汤高(12 g·kg-1)、低(6 g·kg-1)剂量组。除正常组外,其他各组动物经10%卵白蛋白(ovalbumin,OVA)腹腔注射致敏和1%气道吸入激发建立哮喘模型。正常组给予等量生理盐水致敏和治疗。各组分别给予药物治疗后,观察动物肺泡灌洗液(bronchoaveolar lavage fluid,BALF)和外周血中总细胞数、嗜酸性粒细胞(eosinophil,Eos)、淋巴细胞(lymphocyte,Ly)、嗜中性粒细胞(neutrophil,Neu)比率。肺组织病理学主要观察Eos数目及其凋亡情况。结果:模型组动物血中及BALF中Eos,Ly,Neu和总细胞数显著增高,与正常组比较,差异十分显著(P<0.01)。地龙汤高剂量组对外周血及BALF中Eos,Ly,Neu及总细胞数均较模型组有非常显著的差异(P<0.01),显示出与地塞米松组相似的结果;地龙汤低剂量组亦显示出一定的差异性(P<0.05)。模型组支气管黏膜水肿明显,部分支气管内皱褶减少伴部分上皮脱落,支气管壁、管周及肺泡区见炎性细胞浸润,其中较多Eos浸润,支气管壁增厚。电镜下可见模型组细支气管破坏,肺泡Ⅱ型细胞变性,肺泡内Eos浸润。地龙汤高剂量组以上改变显著较轻,并见BALF中Eos凋亡;地龙汤低剂量组改变程度低于高剂量组,并见部分Eos凋亡。结论:地龙汤可减轻哮喘豚鼠气道炎症,其作用与抑制Eos浸润,并促进Eos凋亡有关。作用强弱与剂量呈正比例关系。更多还原

【Abstract】 Objective: To investigate the effect of Earthworm decoction on the airway inflammation of experimental bronchial asthma in guinea pigs and inquire into the mechanism in the decoction.Method: Forty-eight guinea pigs were randomly divided into six groups: the control group,the model group,the dexamethasone group,the Xiaoqinglong decoction group,the earthworm decoction large dosage group and the Earthworm decoction low dosage group,8 guinea pigs in each group.Except the control group,the other groups were sensitized with ovalbumin(OVA) by a combination of intraperitional injection and repeated intranasal challenges to establish the guinea pigs asthma model.However,in the control group,normal saline was used.The morphological changes of bronchial tube,the lung tectology and the inflammation germ cell quantity of eosinophils(Eos),lymphocytes(Ly),neutrophils(Neu) and total blood cells in the blood and bronchoalveolar lavaga fluid(BALF) were examinated in each group respectively.Result: The levels of Eos,Ly,Neu and total cell quantity in the blood and BALF after the earthworm decoction treatment in the large dosage group were significantly lower than those in the model group(P<0.01),and in the low dosage group were lower too(P<0.05).The Earthworm decoction large dosage could obviously improve the bronchial tube epidermis damage,the mucous membrane gland proliferation and hydrops,asthma pathology change and basilar membrane accumulation.Eos apoptosis was obsered in the bronchoalveolar,blood and BALF.The Earthworm decoction small dosage had a similar effect but slightly to the large dosage.Conclusion: The Earthworm decoction can lighten the airway inflammation in asthmatic guinea pigs,its mechanism is related with the inhibition of Eos infiltration,acceleration of Eos apoptosis and improvement of the bronchial tube and the lung tectology changes.The effect of the decoction is dose-dependent.更多还原