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桑白皮中桑辛素的含量测定

Determination of morusin in Cortex Mori

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【作者】 宗玉英叶兆波董婷霞车镇涛

【Author】 ZONG Yu-ying~1 IP Siu-po~1 DONG Ting-xia~2 CHE Chun-tao~1 1.School of Chinese Medicine,The Chinese University of Hongkong,Shatin,Hongkong,China; 2.Department of Biology and Biotechnology Research Institute,Hongkong University of Science and Technology,Clear Water Bay,Hongkong,China

【机构】 香港中文大学中医学院香港科技大学生物系暨生物技术研究所香港中文大学中医学院 香港沙田香港沙田香港九龙

【摘要】 目的:对不同产地,不同品种及规格的桑白皮中的桑辛素进行含量测定。方法:采用高效液相色谱法,Alltima C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水(58:42),检测波长270nm。结果:未经除去黄棕色外皮的桑白皮样品与除去外部黄棕色外皮的样品中桑辛素含量差异明显,样品中的桑辛素主要存在于桑白皮的黄棕色外皮中。桑白皮桑辛素含量与样品厚度与有关。结论:本方法简便,稳定,为桑白皮质量控制提供参考依据。

【Abstract】 Objevtive:To determine the contents of morusin in Sangbaipi(Cortex Mori)of different commercial grades and species and obtained from different regions.Method:Contents of morusin were determined by HPLC on an Alltima C18 column(4.6 mm×250 mm,5μm)with acetonitrile-water(58:42)as mobile phase.UV detection was conduced at 270 nm.Result:The a- mounts of morusin were found to vary among samples of Cortex Mori bearing a yellow-brown outer layer and those witbout the yellow- brown cork layer.The morusin contents were also related to the thickness of Cortex Mori.Conclusion:The method was simple,repro- ducible and reliable.It can be applied to the quality assessment of Cortex Mori.

【关键词】 桑白皮桑辛素高效液相法
【Key words】 Cortex MorimorusinHPLC
  • 【文献出处】 中国中药杂志 ,China Journal of Chinese Materia Medica , 编辑部邮箱 ,2007年11期
  • 【分类号】R284.1
  • 【被引频次】25
  • 【下载频次】726
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